Oligo(dT) primer generates a high frequency of truncated cDNAs through internal poly(A) priming during reverse transcription

We have analyzed a systematic flaw in the current system of gene identification: the oligo(dT) primer widely used for cDNA synthesis generates a high frequency of truncated cDNAs through internal poly(A) priming. Such truncated cDNAs may contribute to 12% of the expressed sequence tags in the curren...

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Detalhes bibliográficos
Main Authors: Nam, Douglas Kyung, Lee, Sanggyu, Zhou, Guolin, Cao, Xiaohong, Wang, Clarence, Clark, Terry, Chen, Jianjun, Rowley, Janet D., Wang, San Ming
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2002
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC122918/
https://ncbi.nlm.nih.gov/pubmed/11972056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.092140899
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