Nalaganje...
Mutations of penicillin acylase residue B71 extend substrate specificity by decreasing steric constraints for substrate binding.
Two mutant forms of penicillin acylase from Escherichia coli strains, selected using directed evolution for the ability to use glutaryl-L-leucine for growth [Forney, Wong and Ferber (1989) Appl. Environ. Microbiol. 55, 2550-2555], are changed within one codon, replacing the B-chain residue Phe(B71)...
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Main Authors: | , , , , , |
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Format: | Artigo |
Jezik: | Inglês |
Izdano: |
2003
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Teme: | |
Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1223260/ https://ncbi.nlm.nih.gov/pubmed/12511194 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20021383 |
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