Engineering of coenzyme specificity of formate dehydrogenase from Saccharomyces cerevisiae.

A eukaryotic formate dehydrogenase (EC 1.2.1.2, FDH) with its substrate specificity changed from NAD(+) to NADP(+) has been constructed by introducing two single-point mutations, Asp(196)-->Ala (D196A) and Tyr(197)-->Arg (Y197R). The mutagenesis was based on the results of homology modelling o...

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Dades bibliogràfiques
Autors principals: Serov, Alexander E, Popova, Anna S, Fedorchuk, Vladimir V, Tishkov, Vladimir I
Format: Artigo
Idioma:Inglês
Publicat: 2002
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1222933/
https://ncbi.nlm.nih.gov/pubmed/12144528
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20020379
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