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Engineering of coenzyme specificity of formate dehydrogenase from Saccharomyces cerevisiae.
A eukaryotic formate dehydrogenase (EC 1.2.1.2, FDH) with its substrate specificity changed from NAD(+) to NADP(+) has been constructed by introducing two single-point mutations, Asp(196)-->Ala (D196A) and Tyr(197)-->Arg (Y197R). The mutagenesis was based on the results of homology modelling o...
Αποθηκεύτηκε σε:
| Κύριοι συγγραφείς: | , , , |
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| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
2002
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1222933/ https://ncbi.nlm.nih.gov/pubmed/12144528 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20020379 |
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