Multiple mutagenesis of non-universal serine codons of the Candida rugosa LIP2 gene and biochemical characterization of purified recombinant LIP2 lipase overexpressed in Pichia pastoris.

The 17 non-universal serine codons (CTG) in the Candida rugosa LIP2 gene have been converted into universal serine codons (TCT) by overlap extension PCR-based multiple site-directed mutagenesis. An active recombinant LIP2 lipase was overexpressed in Pichia pastoris and secreted into the culture medi...

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Hlavní autoři: Lee, Guan-Chiun, Lee, Li-Chiun, Sava, Vasyl, Shaw, Jei-Fu
Médium: Artigo
Jazyk:Inglês
Vydáno: 2002
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Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1222792/
https://ncbi.nlm.nih.gov/pubmed/12020350
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20020404
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