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Cell Surface Display of Thermomyces lanuginosus Lipase in Pichia pastoris

A cell surface displayed system in Pichia pastoris GS115 was developed by using GCW61, a glycosylphosphatidylinositol-modified cell wall protein from P. pastoris, as the anchor protein. Thermomyces lanuginosus lipase (TLL) was successfully displayed on the P. pastoris cell wall by fusing GCW61 gene...

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Detalhes bibliográficos
Publicado no:Front Bioeng Biotechnol
Main Authors: Yang, Jiaming, Huang, Kui, Xu, Xiaomin, Miao, Yangli, Lin, Ying, Han, Shuangyan
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7656992/
https://ncbi.nlm.nih.gov/pubmed/33195113
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.544058
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