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Use of site-directed mutagenesis to probe the structure, function and isoniazid activation of the catalase/peroxidase, KatG, from Mycobacterium tuberculosis.

A series of mutants bearing single amino acid substitutions often encountered in the catalase/peroxidase, KatG, from isoniazid-resistant isolates of Mycobacterium tuberculosis has been produced by site-directed mutagenesis. The resultant enzymes were overexpressed, purified and characterized. Replac...

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Bibliografische gegevens
Hoofdauteurs: Saint-Joanis, B, Souchon, H, Wilming, M, Johnsson, K, Alzari, P M, Cole, S T
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1999
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1220113/
https://ncbi.nlm.nih.gov/pubmed/10051449
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