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Quantification of cathepsins B and L in cells.
A method for quantifying active cysteine proteinases in mammalian cells has been developed using an active-site-directed inhibitor. Fluoren-9-ylmethoxycarbonyl(di-iodotyrosylalanyl)-diaz omethane (Fmoc-[I2]Tyr-Ala-CHN2) was prepared and shown to react irreversibly with cathepsins B and L, but not wi...
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Autores principales: | , , |
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Formato: | Artigo |
Lenguaje: | Inglês |
Publicado: |
1998
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Materias: | |
Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1219506/ https://ncbi.nlm.nih.gov/pubmed/9601080 |
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