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Quantification of cathepsins B and L in cells.

A method for quantifying active cysteine proteinases in mammalian cells has been developed using an active-site-directed inhibitor. Fluoren-9-ylmethoxycarbonyl(di-iodotyrosylalanyl)-diaz omethane (Fmoc-[I2]Tyr-Ala-CHN2) was prepared and shown to react irreversibly with cathepsins B and L, but not wi...

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Detalhes bibliográficos
Main Authors: Xing, R, Addington, A K, Mason, R W
Formato: Artigo
Idioma:Inglês
Publicado em: 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1219506/
https://ncbi.nlm.nih.gov/pubmed/9601080
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