The dependence of DNase I activity on the conformation of oligodeoxynucleotides.

We have developed a sensitive continuous assay for nucleases using proton release. The assay has been applied to the determination of the kinetics of DNase I acting on short, defined deoxyoligonucleotides. The dependence of Kcat/K(m) on sequence and structure of short oligonucleotide substrates has...

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Bibliografiske detaljer
Main Authors: Sutton, D H, Conn, G L, Brown, T, Lane, A N
Format: Artigo
Sprog:Inglês
Udgivet: 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1218094/
https://ncbi.nlm.nih.gov/pubmed/9020884
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