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An in Vitro Assay for Frameshift Mutations: Hotspots for Deletions of 1 Bp by Klenow-Fragment Polymerase Share a Consensus DNA Sequence
The fidelity of in vitro DNA synthesis catalyzed by the large fragment of DNA polymerase I was examined. The templates, specifically designed to detect shifts to the +1 or to the -1 reading frame, are composites of M13mp8 and bacteriophage T4 rIIB DNA and were designed to assist in the identificatio...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1988
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1203272/ https://ncbi.nlm.nih.gov/pubmed/3282984 |
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