Heterologous Expression, Purification, and Immunological Reactivity of a Recombinant HSP60 from Paracoccidioides brasiliensis

The complete coding cDNA of HSP60 from Paracoccidioides brasiliensis was overexpressed in an Escherichia coli host to produce high levels of recombinant protein. The protein was purified by affinity chromatography. A total of 169 human serum samples were tested for reactivity by Western blot analysi...

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Detalhes bibliográficos

Publicado no:	Clin Diagn Lab Immunol
Principais autores:	Cunha, Daniela A., Zancopé-Oliveira, Roseli M., Sueli, M., Felipe, S., Salem-Izacc, Silvia M., Deepe Jr., George S., Soares, Célia M. A.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2002
Assuntos:	Microbial Immunology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC119943/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11874881/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/CDLI.9.2.374-377.2002
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