Heterologous Expression, Purification, and Immunological Reactivity of a Recombinant HSP60 from Paracoccidioides brasiliensis

The complete coding cDNA of HSP60 from Paracoccidioides brasiliensis was overexpressed in an Escherichia coli host to produce high levels of recombinant protein. The protein was purified by affinity chromatography. A total of 169 human serum samples were tested for reactivity by Western blot analysi...

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Podrobná bibliografie

Vydáno v:	Clin Diagn Lab Immunol
Hlavní autoři:	Cunha, Daniela A., Zancopé-Oliveira, Roseli M., Sueli, M., Felipe, S., Salem-Izacc, Silvia M., Deepe Jr., George S., Soares, Célia M. A.
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 2002
Témata:	Microbial Immunology
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC119943/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11874881/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/CDLI.9.2.374-377.2002
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