Heterologous Expression, Purification, and Immunological Reactivity of a Recombinant HSP60 from Paracoccidioides brasiliensis

The complete coding cDNA of HSP60 from Paracoccidioides brasiliensis was overexpressed in an Escherichia coli host to produce high levels of recombinant protein. The protein was purified by affinity chromatography. A total of 169 human serum samples were tested for reactivity by Western blot analysi...

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Dades bibliogràfiques

Publicat a:	Clin Diagn Lab Immunol
Autors principals:	Cunha, Daniela A., Zancopé-Oliveira, Roseli M., Sueli, M., Felipe, S., Salem-Izacc, Silvia M., Deepe Jr., George S., Soares, Célia M. A.
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 2002
Matèries:	Microbial Immunology
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC119943/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11874881/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/CDLI.9.2.374-377.2002
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