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Evidence for histidine in the active site of papain

Papain was irreversibly inhibited by 1,3-dibromoacetone, a reagent designed to react first with the active-site cysteine residue and subsequently with a second nucleophile. The molecular weight of the inhibited enzyme was indistinguishable from that of papain itself, and no evidence of dimeric or ol...

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Detalhes bibliográficos
Main Authors: Husain, S. S., Lowe, G.
Formato: Artigo
Idioma:Inglês
Publicado em: 1968
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1198892/
https://ncbi.nlm.nih.gov/pubmed/5673530
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