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Determination of the folding transition states of barnase by using Φ(I)-value-restrained simulations validated by double mutant Φ(IJ)-values

The protein barnase folds from the denatured state into its native conformation via a high-energy intermediate. Using Φ(I)-values determined experimentally from single-point mutations as restraints in all-atom molecular dynamics simulations, we have determined ensembles of structures corresponding t...

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Hlavní autoři: Salvatella, Xavier, Dobson, Christopher M., Fersht, Alan R., Vendruscolo, Michele
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2005
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1194897/
https://ncbi.nlm.nih.gov/pubmed/16116084
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0408226102
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