Development of a Plasmid-Mediated Reporter System for In Vivo Monitoring of Gene Expression in the Archaeon Methanosarcina acetivorans

A plasmid-based gene reporter system has been developed to construct lacZ gene fusions for monitoring intrinsic promoter expression in Methanosarcina acetivorans. Constructs transform with high efficiency that can be readily screened by color selection on plates and exhibit a consistent copy number...

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Bibliographic Details
Main Authors: Apolinario, Ethel E., Jackson, Karin M., Sowers, Kevin R.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2005
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Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1183294/
https://ncbi.nlm.nih.gov/pubmed/16085896
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.8.4914-4918.2005
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