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Development of a Plasmid-Mediated Reporter System for In Vivo Monitoring of Gene Expression in the Archaeon Methanosarcina acetivorans

A plasmid-based gene reporter system has been developed to construct lacZ gene fusions for monitoring intrinsic promoter expression in Methanosarcina acetivorans. Constructs transform with high efficiency that can be readily screened by color selection on plates and exhibit a consistent copy number...

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Autori principali: Apolinario, Ethel E., Jackson, Karin M., Sowers, Kevin R.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1183294/
https://ncbi.nlm.nih.gov/pubmed/16085896
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.8.4914-4918.2005
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