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Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication

We demonstrate a new method for making oligonucleotide microarrays by synthesis in situ. The method uses conventional DNA synthesis chemistry with an electrochemical deblocking step. Acid is delivered to specific regions on a glass slide, thus allowing nucleotide addition only at chosen sites. The a...

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Autori principali: Egeland, Ryan D., Southern, Edwin M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1183109/
https://ncbi.nlm.nih.gov/pubmed/16085751
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni117
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