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Efficient bacterial transcription of DNA nanocircle vectors with optimized single-stranded promoters

We describe experiments aimed at establishing whether circular single-stranded DNAs can form promoters for bacterial transcription from small folded motifs. In vitro selection experiments were carried out on circular 103-nt DNA libraries encoding 40-nt randomized sequences as well as self-processing...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Ohmichi, Tatsuo, Maki, Angèle, Kool, Eric T.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The National Academy of Sciences 2002
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC117513/
https://ncbi.nlm.nih.gov/pubmed/11752404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.012589099
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