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Measurements of weak interactions between truncated substrates and a hammerhead ribozyme by competitive kinetic analyses: implications for the design of new and efficient ribozymes with high sequence specificity

Exploitation of ribozymes in a practical setting requires high catalytic activity and strong specificity. The hammerhead ribozyme R32 has considerable potential in this regard since it has very high catalytic activity. In this study, we have examined how R32 recognizes and cleaves a specific substra...

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Auteurs principaux: Kasai, Yasuhiro, Shizuku, Hideki, Takagi, Yasuomi, Warashina, Masaki, Taira, Kazunari
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2002
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC117203/
https://ncbi.nlm.nih.gov/pubmed/12034825
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