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Dynamics and efficiency in vivo of UGA-directed selenocysteine insertion at the ribosome.
The kinetics and efficiency of decoding of the UGA of a bacterial selenoprotein mRNA with selenocysteine has been studied in vivo. A gst-lacZ fusion, with the fdhF SECIS element ligated between the two fusion partners, gave an efficiency of read-through of 4-5%; overproduction of the selenocysteine...
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1999
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1171311/ https://ncbi.nlm.nih.gov/pubmed/10205181 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/18.8.2284 |
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