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Characterization of the iron- and 2-oxoglutarate-binding sites of human prolyl 4-hydroxylase.
Prolyl 4-hydroxylase (EC 1.14.11.2), an alpha2beta2 tetramer, catalyzes the formation of 4-hydroxyproline in collagens. We converted 16 residues in the human alpha subunit individually to other amino acids, and expressed the mutant polypeptides together with the wild-type beta subunit in insect cell...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1169716/ https://ncbi.nlm.nih.gov/pubmed/9135134 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/16.6.1173 |
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