Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast pichia pastoris: formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen requires coexpression with prolyl 4-hydroxylase.

Prolyl 4-hydroxylase, the key enzyme of collagen synthesis, is an alpha2beta2 tetramer, the beta subunit of which is protein disulfide isomerase (PDI). Coexpression of the human alpha subunit and PDI in Pichia produced trace amounts of an active tetramer. A much higher, although still low, assembly...

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Bibliographic Details
Main Authors: Vuorela, A, Myllyharju, J, Nissi, R, Pihlajaniemi, T, Kivirikko, K I
Format: Artigo
Language:Inglês
Published: 1997
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1170275/
https://ncbi.nlm.nih.gov/pubmed/9362485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/16.22.6702
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