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Inosine 5'-monophosphate dehydrogenase of Escherichia coli. Purification by affinity chromatography, subunit structure and inhibition by guanosine 5'-monophosphate.
Escherichia coli IMP dehydrogenase (EC 1.2.1.14) was purified by affinity chromatography on immobilized nucleotides. The enzyme binds to agarose-bound 8-(6-aminohexyl)-AMP, N6-(6-aminohexyl)-AMP and 8-(8-amino-octyl)-IMP but not to immobilized NAD+ or Cibacron Blue F3G-A. AMP proved to be an effecti...
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Main Authors: | , , |
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格式: | Artigo |
語言: | Inglês |
出版: |
1979
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主題: | |
在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1161627/ https://ncbi.nlm.nih.gov/pubmed/44191 |
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