Comparison of Six Methods of Extracting Mycobacterium tuberculosis DNA from Processed Sputum for Testing by Quantitative Real-Time PCR

Six methods of extracting Mycobacterium tuberculosis DNA from sputum for testing by quantitative PCR were compared: Tris-EDTA (TE) buffer, PrepMan Ultra, 2% sodium dodecyl sulfate (SDS)-10% Triton X with and without sonication, Infectio Diagnostics, Inc. (IDI) lysing tubes, and QIAGEN QIAamp DNA min...

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Detalhes bibliográficos
Main Authors: Aldous, Wade K., Pounder, June I., Cloud, Joann L., Woods, Gail L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Mycobacteriology and Aerobic Actinomycetes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1153782/
https://ncbi.nlm.nih.gov/pubmed/15872286
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.5.2471-2473.2005
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