Use of the MGB Eclipse System and SmartCycler PCR for Differentiation of Mycobacterium chelonae and M. abscessus

Although accurate in the identification of Mycobacterium species, partial 16S rRNA gene sequencing does not distinguish Mycobacterium chelonae from M. abscessus. Thus, we designed a SmartCycler PCR assay targeting the 16S-to-23S internal transcribed spacer (ITS) region with use of MGB Eclipse probes...

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Bibliographic Details
Main Authors: Cloud, Joann L., Hoggan, Karen, Belousov, Evgeniy, Cohen, Samuel, Brown-Elliott, Barbara A., Mann, Linda, Wilson, Rebecca, Aldous, Wade, Wallace, Richard J., Woods, Gail L.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2005
Subjects:
Mycobacteriology and Aerobic Actinomycetes
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1233948/
https://ncbi.nlm.nih.gov/pubmed/16081979
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.8.4205-4207.2005
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