Direct Detection of Rifampin- and Isoniazid-Resistant Mycobacterium tuberculosis in Auramine-Rhodamine-Positive Sputum Specimens by Real-Time PCR

Our objective was to evaluate the feasibility of a molecular assay based on a real-time PCR technique, carried out with a LightCycler instrument (Roche Biochemicals), to identify Mycobacterium tuberculosis bacilli and to detect rifampin and isoniazid resistance in DNA extracts from sputum samples. W...

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Detalhes bibliográficos
Main Authors: Ruiz, Maite, Torres, Maria J., Llanos, Ana C., Arroyo, Aurelio, Palomares, Jose C., Aznar, Javier
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Mycobacteriology and Aerobic Actinomycetes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC387553/
https://ncbi.nlm.nih.gov/pubmed/15071008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.4.1585-1589.2004
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