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An experimental method to determine the substrate protection of enzyme against deactivation in a reversible reaction.
The substrate protection effect on an enzyme in a reversible reaction was studied by using glucose isomerase immobilized in small particles (radius less than 100 micron). Deactivation of the enzyme at various substrate concentrations in Tris buffer, pH 8.25, at 62.1 degrees C was studied in eight-co...
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Format: | Artigo |
Jezik: | Inglês |
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1986
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Teme: | |
Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1146880/ https://ncbi.nlm.nih.gov/pubmed/3753468 |
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