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Purification of xyloglucan endotransglycosylases (XETs): a generally applicable and simple method based on reversible formation of an enzyme-substrate complex.

We describe a novel and general, mechanism-based, method for purification of xyloglucan endotransglycosylases (XETs) from crude plant extracts. Putative isoforms, obtained by step-wise precipitation with (NH4)2SO4, were incubated with tamarind xyloglucan (approximately 1 MDa) to form stable xylogluc...

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Main Authors: Steele, N M, Fry, S C
Format: Artigo
Jezik:Inglês
Izdano: 1999
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1220239/
https://ncbi.nlm.nih.gov/pubmed/10229676
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