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Action of intact AP (apurinic/apyrimidinic) sites and AP sites associated with breaks on the transcription of T7 coliphage DNA by Escherichia coli RNA polymerase.

The effect of apurinic/apyrimidinic (AP) sites in DNA on RNA and protein synthesis was studied in vitro using T7 coliphage DNA. Initiation of RNA synthesis by Escherichia coli RNA polymerase was synchronized and heparin was used to prevent reinitiation. When the T7 DNA contained AP sites, the rate o...

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Hlavní autoři: Flamée, P A, Verly, W G
Médium: Artigo
Jazyk:Inglês
Vydáno: 1985
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1145164/
https://ncbi.nlm.nih.gov/pubmed/2412545
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