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The use of thioglycolate to distinguish between 3' AP (apurinic/apyrimidinic) endonucleases and AP lyases.

Addition of thioglycolate and DEAE-Sephadex chromatography were used to analyze the cleavage of the C(3')-O-P bond 3' to AP (apurinic/apyrimidinic) sites in DNA and to distinguish between a mechanism of hydrolysis (which would allow the nicking enzyme to be called 3' AP endonuclease)...

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Main Authors: Bricteux-Grégoire, S, Verly, W G
Format: Artigo
Sprog:Inglês
Udgivet: 1989
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC318277/
https://ncbi.nlm.nih.gov/pubmed/2475855
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