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In Vitro Assembly of PB2 with a PB1-PA Dimer Supports a New Model of Assembly of Influenza A Virus Polymerase Subunits into a Functional Trimeric Complex

Influenza virus RNA-dependent RNA polymerase is a heterotrimeric complex of PB1, PB2, and PA. We show that the individually expressed PB2 subunit can be assembled with the coexpressed PB1-PA dimer in vitro into a transcriptionally active complex. Furthermore, we demonstrate that a model viral RNA pr...

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Detalhes bibliográficos
Main Authors: Deng, Tao, Sharps, Jane, Fodor, Ervin, Brownlee, George G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1143706/
https://ncbi.nlm.nih.gov/pubmed/15956611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.79.13.8669-8674.2005
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