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Proximity of the U12 snRNA with both the 5′ Splice Site and the Branch Point during Early Stages of Spliceosome Assembly

U12 snRNA is required for branch point recognition in the U12-dependent spliceosome. Using site-specific cross-linking, we have captured an unexpected interaction between the 5′ end of the U12 snRNA and the −2 position upstream of the 5′ splice site of P120 and SCN4a splicing substrates. The U12 snR...

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Detalhes bibliográficos
Main Authors: Frilander, Mikko J., Meng, Xiaojuan
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1140575/
https://ncbi.nlm.nih.gov/pubmed/15923601
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.25.12.4813-4825.2005
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