Detection of rpoB Mutations Associated with Rifampin Resistance in Mycobacterium tuberculosis Using Denaturing Gradient Gel Electrophoresis

Denaturing gradient gel electrophoresis (DGGE) was used to probe for mutations associated with rifampin (RIF) resistance in the rpoB gene of Mycobacterium tuberculosis. DGGE scans for mutations across large regions of DNA and is comparable to DNA sequencing in detecting DNA alterations. Specific mut...

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Detalhes bibliográficos
Main Authors: McCammon, Mark T., Gillette, John S., Thomas, Derek P., Ramaswamy, Srinivas V., Graviss, Edward A., Kreiswirth, Barry N., Vijg, Jan, Quitugua, Teresa N.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Mechanisms of Resistance
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1140537/
https://ncbi.nlm.nih.gov/pubmed/15917513
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.49.6.2200-2209.2005
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