End-specific covalent photo-dependent immobilisation of synthetic DNA to paramagnetic beads
A novel approach for light-dependent covalent immobilisation of synthetic DNA oligomers to amino-coated paramagnetic beads is described. A hetero-bifunctional photo-reactive cross-linking chemical, 4-nitrophenyl 3-diazopyruvate, is applied to attach 5′ amino-modified DNA to both silica and polystyre...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC113890/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11071952/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.22.e98 |
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