End-specific covalent photo-dependent immobilisation of synthetic DNA to paramagnetic beads

A novel approach for light-dependent covalent immobilisation of synthetic DNA oligomers to amino-coated paramagnetic beads is described. A hetero-bifunctional photo-reactive cross-linking chemical, 4-nitrophenyl 3-diazopyruvate, is applied to attach 5′ amino-modified DNA to both silica and polystyre...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Penchovsky, Robert, Birch-Hirschfeld, E., McCaskill, John S.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2000
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC113890/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11071952/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.22.e98
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