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Synthetic, structural and biological studies of the ubiquitin system: the total chemical synthesis of ubiquitin.

The small protein ubiquitin (76 amino acids) has been synthesized under optimized conditions by Merrifield solid-phase methodology using the N alpha-Fmoc protecting group. The crude polypeptide mixture was purified to homogeneity by gel filtration, dialysis and a combination of cation- and anion-exc...

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Bibliographische Detailangaben
Hauptverfasser: Ramage, R, Green, J, Muir, T W, Ogunjobi, O M, Love, S, Shaw, K
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1994
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1138034/
https://ncbi.nlm.nih.gov/pubmed/8166633
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