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Characterization of human DNA ligase I expressed in a baculovirus-insect cell system.

The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 10(8) Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombin...

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Detalhes bibliográficos
Main Authors: Gallina, A, Rossi, F, Milanesi, G, Rossi, R, Montecucco, A, Ciarrocchi, G
Formato: Artigo
Idioma:Inglês
Publicado em: 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1136303/
https://ncbi.nlm.nih.gov/pubmed/8526875
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