Characterization of human DNA ligase I expressed in a baculovirus-insect cell system.

The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 10(8) Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombin...

Full description

Saved in:
Bibliographic Details
Main Authors: Gallina, A, Rossi, F, Milanesi, G, Rossi, R, Montecucco, A, Ciarrocchi, G
Format: Artigo
Language:Inglês
Published: 1995
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1136303/
https://ncbi.nlm.nih.gov/pubmed/8526875
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items