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Expression, purification, characterization and structure of Pseudomonas aeruginosa arylamine N-acetyltransferase
The gene for NAT (arylamine N-acetyltransferase) from Pseudomonas aeruginosa (panat) has been cloned from genomic DNA, and the gene product (PANAT) expressed as an N-terminal histidine-tagged protein in Escherichia coli and purified via nickel ion affinity chromatography. The specific activities of...
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Main Authors: | , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Portland Press Ltd.
2005
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1134735/ https://ncbi.nlm.nih.gov/pubmed/15447630 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20041330 |
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