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Expression, purification, characterization and structure of Pseudomonas aeruginosa arylamine N-acetyltransferase

The gene for NAT (arylamine N-acetyltransferase) from Pseudomonas aeruginosa (panat) has been cloned from genomic DNA, and the gene product (PANAT) expressed as an N-terminal histidine-tagged protein in Escherichia coli and purified via nickel ion affinity chromatography. The specific activities of...

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Detalhes bibliográficos
Main Authors: Westwood, Isaac M., Holton, Simon J., Rodrigues-Lima, Fernando, Dupret, Jean-Marie, Bhakta, Sanjib, Noble, Martin E. M., Sim, Edith
Formato: Artigo
Idioma:Inglês
Publicado em: Portland Press Ltd. 2005
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1134735/
https://ncbi.nlm.nih.gov/pubmed/15447630
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20041330
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