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Secondary structure, conformational stability and glycosylation of a recombinant Candida rugosa lipase studied by Fourier-transform infrared spectroscopy

The secondary structure of lipase 1 from Candida rugosa, a model system for large monomeric enzymes, has been studied by FTIR (Fourier-transform infrared) spectroscopy in water and (2)H(2)O. The secondary structure content, determined by the analysis of the amide I band absorption through second der...

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Detalhes bibliográficos
Main Authors: Natalello, Antonino, Ami, Diletta, Brocca, Stefania, Lotti, Marina, Doglia, Silvia M.
Formato: Artigo
Idioma:Inglês
Publicado em: Portland Press Ltd. 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1134723/
https://ncbi.nlm.nih.gov/pubmed/15362976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20041296
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