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High-level expression and in vitro mutagenesis of a fibrillogenic 109-amino-acid C-terminal fragment of Alzheimer's-disease amyloid precursor protein.

We amplified DNA encoding the 3' 109 codons of Alzheimer's-disease amyloid precursor protein (APP) inclusive of the beta protein (A beta) and cytoplasmic domains from cDNA using oligonucleotide primers designed to facilitate cloning into the T7 expression vector pT7Ad23K13. We also modifie...

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Bibliografiske detaljer
Main Authors: Gardella, J E, Gorgone, G A, Candela, L, Ghiso, J, Castaño, E M, Frangione, B, Gorevic, P D
Format: Artigo
Sprog:Inglês
Udgivet: 1993
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1134514/
https://ncbi.nlm.nih.gov/pubmed/8379923
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