Importance of the two tryptophan residues in the Streptomyces R61 exocellular DD-peptidase.

Modification of the Streptomyces R61 DD-peptidase by N-bromosuccinimide resulted in a rapid loss of enzyme activity. In consequence, the role of the enzyme's two tryptophan residues was investigated by site-directed mutagenesis. Trp271 was replaced by Leu. The modification yielded a stable enzy...

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Detalhes bibliográficos
Main Authors: Bourguignon-Bellefroid, C, Wilkin, J M, Joris, B, Aplin, R T, Houssier, C, Prendergast, F G, Van Beeumen, J, Ghuysen, J M, Frère, J M
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1130787/
https://ncbi.nlm.nih.gov/pubmed/1546952
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