Mutational Analysis of Bovine Viral Diarrhea Virus RNA-Dependent RNA Polymerase

Recombinant bovine viral diarrhea virus (BVDV) nonstructural protein 5B (NS5B) produced in insect cells has been shown to possess an RNA-dependent RNA polymerase (RdRp) activity. Our initial attempt to produce the full-length BVDV NS5B with a C-terminal hexahistidine tag in Escherichia coli failed d...

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Pubblicato in:	J Virol
Autori principali:	Lai, Vicky C. H., Kao, C. Cheng, Ferrari, Eric, Park, Justin, Uss, Annette S., Wright-Minogue, Jacquelyn, Hong, Zhi, Lau, Johnson Y. N.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1999
Soggetti:	Replication
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC113065/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10559328/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.73.12.10129-10136.1999
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