Mutational Analysis of Bovine Viral Diarrhea Virus RNA-Dependent RNA Polymerase

Recombinant bovine viral diarrhea virus (BVDV) nonstructural protein 5B (NS5B) produced in insect cells has been shown to possess an RNA-dependent RNA polymerase (RdRp) activity. Our initial attempt to produce the full-length BVDV NS5B with a C-terminal hexahistidine tag in Escherichia coli failed d...

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Autori principali: Lai, Vicky C. H., Kao, C. Cheng, Ferrari, Eric, Park, Justin, Uss, Annette S., Wright-Minogue, Jacquelyn, Hong, Zhi, Lau, Johnson Y. N.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 1999
Soggetti:
Replication
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC113065/
https://ncbi.nlm.nih.gov/pubmed/10559328
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