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Offset recombinant PCR: a simple but effective method for shuffling compact heterologous domains

DNA shuffling and other in vitro recombination strategies have proven highly effective at generating complex libraries for mutagenesis studies. While most recombination techniques employ DNA polymerases in part of a multi-step process, few seek to exploit the natural recombinogenic tendencies and ex...

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Détails bibliographiques
Auteurs principaux: Rozak, David A., Bryan, Philip N.
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2005
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1130489/
https://ncbi.nlm.nih.gov/pubmed/15901853
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni081
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