Use of Real-Time PCR and Molecular Beacons To Detect Virus Replication in Human Immunodeficiency Virus Type 1-Infected Individuals on Prolonged Effective Antiretroviral Therapy

We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (W...

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Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Lewin, S. R., Vesanen, M., Kostrikis, L., Hurley, A., Duran, M., Zhang, L., Ho, D. D., Markowitz, M.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Pathogenesis and Immunity
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC112674/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10364365/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.73.7.6099-6103.1999
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