Use of Real-Time PCR and Molecular Beacons To Detect Virus Replication in Human Immunodeficiency Virus Type 1-Infected Individuals on Prolonged Effective Antiretroviral Therapy

We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (W...

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Main Authors: Lewin, S. R., Vesanen, M., Kostrikis, L., Hurley, A., Duran, M., Zhang, L., Ho, D. D., Markowitz, M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1999
Assuntos:
Pathogenesis and Immunity
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC112674/
https://ncbi.nlm.nih.gov/pubmed/10364365
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