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Affinity-Tagged Miniprion Derivatives Spontaneously Adopt Protease-Resistant Conformations

An abridged PrP molecule of 106 amino acids designated PrP106 can form infectious miniprions in transgenic (Tg) mice (29). Addition of six-histidine (His(6)) affinity tags to selective sites within PrP106 resulted unexpectedly in new PrP proteins that spontaneously adopted protease-resistant conform...

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Publicado no:J Virol
Principais autores: Supattapone, Surachai, Nguyen, Hoang-Oanh B., Muramoto, Tamaki, Cohen, Fred E., DeArmond, Stephen J., Prusiner, Stanley B., Scott, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2000
Assuntos:
Structure and Assembly
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC112476/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11090193/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.74.24.11928-11934.2000
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