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Purification and Characterization of the Alanine Aminotransferase from the Hyperthermophilic Archaeon Pyrococcus furiosus and Its Role in Alanine Production

Alanine aminotransferase (AlaAT) was purified from cell extracts of the hyperthermophilic archaeon Pyrococcus furiosus by multistep chromatography. The enzyme has an apparent molecular mass of 93.5 kDa, as estimated by gel filtration, and consists of two identical subunits of 46 kDa, as deduced by s...

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Detaylı Bibliyografya
Yayımlandı:J Bacteriol
Asıl Yazarlar: Ward, Donald E., Kengen, Servé W. M., van der Oost, John, de Vos, Willem M.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology (ASM) 2000
Konular:
Online Erişim:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC111321/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10762259/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.9.2559-2566.2000
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